پاورپوینت Photosynthesi The Hill Reaction (pptx) 24 اسلاید
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Photosynthesis II: The Hill Reaction
What’s the point of this lab?
Understand the electron transport chain
Alter variables in the ETC to observe outcome
Develop methods to determine order of events in the ETC
Reminders
6CO2 + 12H2O + light C6H12O6 + 6O2 + 6H2O
Excitation Chlorophyll (water split) ETC (PQ as H+ pump) Excitation Chlorophyll ETC Reduction NADP+ to NADPH + H+ (stroma)
High H+ concentration in thylakoid interior causes ATP production (stroma)
Electron Transport Chain
DCPIP – the electron theif
High absorbance at 600 nm
Blue = Oxidized (electron poor)
Clear = Reduced (electron rich)
Hill Reaction (Figure 7.1)
Review of Hill Reaction
Isolated chloroplasts could be stimulated by light to transfer e- through ETC to an artificial electron acceptor
We measure the Hill Reaction with other e- acceptors
How do herbicides affect the ETC?
DCPIP: artificial e- acceptor from ETC
Herbicide: blocks ETC
Hill Reaction
http://www.life.uiuc.edu/govindjee/photoweb/NewZScheme.jpg
DCPIP
(Hill Reaction)
DCPIP
Blue
Oxidized (no e-)
Colorless
Reduced (gains e-)
If Photosystem II is not stopped, and DCPIP is allowed to steal electrons and unclog the e- back-up in the ETC, then H2O is split into 2 H+ and O2 and e-'s are moved down the chain.
Electron Transport Chain